1 kb ladder invitrogen pdf free

The product is supplied in a solution of 10 mm trishcl, ph 8. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm tris ph 7. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The 1 kb dna ladder contains discrete dna fragments ranging from 300 bp to. Trackit 1 kb plus dna ladder thermo fisher scientific. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. A single convenient ladder for 100 bp up to 10 kb 100 bp and 1 kb ladder in one. Supercoiled dna ladder from invitrogen biomedicine. The bands of the ladder contain fragments with the. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. It allows sizing and concentration estimate of dna fragments on agarose gels generated by pcr or restriction digest. Identification of the substancemixture and of the companyundertaking product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable.

View more information about our dna stains technology. The ladder is supplied in gel loading buffer and can be applied directly to the gel. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. How can i prepare working solution with 1 kb dna ladder. Generuler 1 kb dna ladder thermo fisher scientific. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. Use the neb 1 kb ladder for size markers between 500 bp and 10 kb. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb.

This ladder consists of bands containing 1 8 repeats of a 1018bp dna fragment. Every readytouse ladder is supplied with the nucleasefree loading dye solution, which ensures optimal migration and quantification of your dna probes. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. Readyload 1 kb p dna ladder thermo fisher scientific. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. The 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 0.

The 10 bands of the ladder contain fragments following sizes. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. Invitrogen 1kb dna extension ladder 100ug nucleic acid. Invitrogen cell and molecular biology products range from doityourself reagents and optimized kits to connected instruments, all working together holistically to. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Readytorun dna digests and ladder allow you to pipet samples into the wells of an agarose gel and run the electrophoresis apparatus. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. The bands can be visualized by ethidium bromide staining and may also be detected on southern blots by probing with the pbr322 betalactamase ampicillinresistance gene sequence. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Making 1kb ladder working solution university of san diego.

The 1 kb plus dna ladder for safe stains is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The digested dna includes fragments ranging from 0. Neb also offers a quickload version of this ladder with purple dye. All rna molecules in the ladder are polyatailed 40 nt for convenient use as control templates for cdna synthesis. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel. How to dilute dna ladders andersen lab, 2016 invitrogen 1. If samples need to be diluted, use te or other buffer of minimal ionic strength. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp.

The 1 kb dna ladder has a number of proprietary plasmids that are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Supercoiled dna ladder from invitrogen,supercoiled dna ladder is suitable for sizing supercoiled dna from 2 to 16 kb by agarose gel electrophoresis 1. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. This marker is ideal for the size determination of digested dna. The ladder is composed of fourteen chromatographypurified. Readyload 1 kb plus dna ladder is intended for use as a molecular weight standard in agarose and polyacrylamide gel electrophoresis. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Use the invitrogen 1 kb plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds.

The 1 kb plus dna ladder for safe stains might be used on sybr green i precast agarose gels but might require further dilution and optimization. The 1 kb dna ladder is a readytoload molecular weight marker suitable for. For optimal resolution, the recommended agarose gel concentration is 0. The 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 250 bp to 10 kb.